Uracil-DNA glycosylase


Enzyme for prevention of false positive results in PCR

Cat.N

Size, U

Size, ml

Price

E-UDG-50

50 000

5 ml

1320 EUR

Benefits:

  • Inactivated during the first PCR cycle
  • Prevents false-positive results
  • Maximal activity at 37 °C

Concentration:
10 units/μl

Area of applications:

  • Routine PCR
  • Multiplex PCR
  • qPCR with dual-labeled probes
  • Real-time PCR with intercalating dyes

Storage and transportation conditions:

from -24°С to -16°С — 2 years

up to 14 days at a temperature <25°С

Uracil-DNA-glycosylase is an enzyme that catalyzes the hydrolysis of the N-glycosyl bond between uracil and the sugar residue in single- and double-stranded DNA in the presence of dUTP residues in a DNA chain. The enzyme possesses maximal activity at 37 °C. Uracil-DNA glycosylase is used in the preparation of PCR mixtures to prevent the appearance of false-positive results caused by contamination of the PCR reaction mixture with products of previously performed PCR (amplicons), which are destroyed before amplification. The enzyme is not active against RNA and short oligonucleotides. The enzyme inactivated completely during the first cycle of DNA denaturation and does not interfere with the amplification of the products of the current reaction.

Area of applications Routine PCR
Multiplex PCR
qPCR with dual-labeled probes
Real-time PCR with intercalating dyes
Concentration 10 units/μl
Storage conditions from -24°С to -16°С — 2 years
Transportation conditions up to 14 days at a temperature <25°С

E-UDG-50

1320 EUR

Size:
50 000 U


Delivery takes up to
5-12 business days