Москва, ул.Годовикова 
M-MLV Reverse Transcriptase
RevM revertase
M-MLV Reverse Transcriptase
Cat. No.
Size, U
Size, ml
Price
To order
* supplied with 10x PCR buffer and 50 mM MgCl2
Benefits:
- Optimum working temperature 55 °C
- Amplification of long PCR fragments up to 12000 bp
- Effective for GC-rich RNA templates
- High productivity
Area of applications:
- Synthesis of cDNA on RNA template
- RT-PCR
- One step RT-PCR
- Preparation of cDNA libraries
Storage and dilution buffer: 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50 % glycerol, 0.5 % Tween-20, 1 mM TCEP
Concentration:
200 units/μl
Storage and transportation conditions:
from -24°С to -16°С – 2 years
from + 2°С to + 8°С – 3 months
up to 14 days at a temperature <25°С
RevM is a genetically modified reverse transcriptase of Moloney Mouse Leukemia Virus (MMLV) which performs the synthesis of a complementary strand of DNA (cDNA) on a singlestranded RNA template. Due to the presence of several mutations, the enzyme possesses inhibited RNase H activity and has increased thermostability at below 65°C, with an optimal temperature of 50°C. Compared to wild-type MMLV, our enzyme provides higher cDNA yield, allows longer long (up to 12 kbp) fragments amplification and has higher productivity with GC-rich RNA templates.
| Area of applications | Synthesis of cDNA on RNA template RT-PCR One step RT-PCR Preparation of cDNA libraries |
| Concentration | 5 units/μl |
| Storage conditions | from -24°С to -16°С – 2 years from + 2°С to + 8°С – 3 months |
| Transportation conditions | up to 14 days at a temperature <25°С |
| Storage and dilution buffer | 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50 % glycerol, 0.5 % Tween-20 |