RevM revertase


M-MLV Reverse Transcriptase

Cat. No.

Size, U

Size, ml

Price

To order

E-RT-50

50 000 Е.А.

10 ml

650 EUR

E-RT-50B*

50 000 Е.А.

10 ml

1 950 EUR

E-RT-500

500 000 Е.А.

100 ml

5 200 EUR

E-RT-500B*

500 000 Е.А.

100 ml

11 850 EUR

* supplied with 10x PCR buffer and 50 mM MgCl2

Benefits:

  • Optimum working temperature 55 °C
  • Amplification of long PCR fragments up to 12000 bp
  • Effective for GC-rich RNA templates
  • High productivity

Area of applications:

  • Synthesis of cDNA on RNA template
  • RT-PCR
  • One step RT-PCR
  • Preparation of cDNA libraries

Storage and dilution buffer: 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50 % glycerol, 0.5 % Tween-20, 1 mM TCEP

Concentration:
200 units/μl

Storage and transportation conditions:

from -24°С to -16°С – 2 years
from + 2°С to + 8°С – 3 months

up to 14 days at a temperature <25°С

RevM is a genetically modified reverse transcriptase of Moloney Mouse Leukemia Virus (MMLV) which performs the synthesis of a complementary strand of DNA (cDNA) on a singlestranded RNA template. Due to the presence of several mutations, the enzyme possesses inhibited RNase H activity and has increased thermostability at below 65°C, with an optimal temperature of 50°C. Compared to wild-type MMLV, our enzyme provides higher cDNA yield, allows longer long (up to 12 kbp) fragments amplification and has higher productivity with GC-rich RNA templates.

Area of applications Synthesis of cDNA on RNA template
RT-PCR
One step RT-PCR
Preparation of cDNA libraries
Concentration 5 units/μl
Storage conditions from -24°С to -16°С – 2 years
from + 2°С to + 8°С – 3 months
Transportation conditions up to 14 days at a temperature <25°С
Storage and dilution buffer 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA,
100 mМ KCl, 50 % glycerol, 0.5 % Tween-20

E-RT-50

650 EUR

Size :
50 000 U


Delivery takes up to
5-12 business days

E-RT-500

5 200 EUR

Size :
500 000 U


Delivery takes up to
5-12 business days