Москва, ул.Годовикова 
Revm hot start revertase en
RevM Hot Start Revertase
M-MLV Reverse Transcriptase with hot start
Cat. No.
Size, U
Size, ml
Price
To order
* supplied with 10x PCR buffer and 50 mM MgCl2
Benefits:
- Inactive at temperatures below 50 °С
- Optimum working temperature 55 °C
- Amplification of long PCR fragments up to 12000 bp
- Effective for GC-rich RNA templates
- High productivity
Area of applications:
- Synthesis of cDNA on RNA template
- RT-PCR
- One step RT-PCR
- Preparation of cDNA libraries
Storage and dilution buffer: 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50 % glycerol, 0.5 % Tween-20, 1 mM TCEP
Concentration:
5 units/μl
Storage and transportation conditions:
from -24°С to -16°С – 2 years
from + 2°С to + 8°С – 3 months
up to 14 days at a temperature <25°С
RevM Hot Start Revertase is a genetically modified reverse transcriptase of Moloney mouse leukemia virus (MMLV), is intended for the synthesis of a complementary DNA strand (cDNA) on a single-stranded RNA template. Due to the presence of several mutations in the enzyme possesses inhibited RNase H activity and has increased thermostability. The enzyme remains inactive at room temperature. It activates at 50 °C and have an optimal working temperature of 55 °C. Inactivation occurs after 10 minutes at 80 °C. Compared to wild-type MMLV, reverse transcriptase RevM provides a higher yield of cDNA, allows the amplification of longer fragments (up to 12,000 bp), has improved efficiency when using GC-rich RNA templates, and also has a higher productivity.
| Area of applications | Synthesis of cDNA on RNA template RT-PCR One step RT-PCR Preparation of cDNA libraries |
| Concentration | 5 units/μl |
| Storage conditions | from -24°С to -16°С – 2 years from + 2°С to + 8°С – 3 months |
| Transportation conditions | up to 14 days at a temperature <25°С |
| Storage and dilution buffer | 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50 % glycerol, 0.5 % Tween-20 |