RevM Hot Start Revertase


M-MLV Reverse Transcriptase with hot start

Cat. No.

Size, U

Size, ml

Price

To order

E-RTH-50

50 000 Е.А.

10 ml

650 EUR

E-RTH-50B*

50 000 Е.А.

10 ml

1 950 EUR

E-RTH-500

500 000 Е.А.

100 ml

5 200 EUR

E-RTH-500B*

500 000 Е.А.

100 ml

11 850 EUR

* supplied with 10x PCR buffer and 50 mM MgCl2

Benefits:

  • Inactive at temperatures below 50 °С
  • Optimum working temperature 55 °C
  • Amplification of long PCR fragments up to 12000 bp
  • Effective for GC-rich RNA templates
  • High productivity

Area of applications:

  • Synthesis of cDNA on RNA template
  • RT-PCR
  • One step RT-PCR
  • Preparation of cDNA libraries

Storage and dilution buffer: 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50 % glycerol, 0.5 % Tween-20, 1 mM TCEP

Concentration:
5 units/μl

Storage and transportation conditions:

from -24°С to -16°С – 2 years
from + 2°С to + 8°С – 3 months

up to 14 days at a temperature <25°С

RevM Hot Start Revertase is a genetically modified reverse transcriptase of Moloney mouse leukemia virus (MMLV), is intended for the synthesis of a complementary DNA strand (cDNA) on a single-stranded RNA template. Due to the presence of several mutations in the enzyme possesses inhibited RNase H activity and has increased thermostability. The enzyme remains inactive at room temperature. It activates at 50 °C and have an optimal working temperature of 55 °C. Inactivation occurs after 10 minutes at 80 °C. Compared to wild-type MMLV, reverse transcriptase RevM provides a higher yield of cDNA, allows the amplification of longer fragments (up to 12,000 bp), has improved efficiency when using GC-rich RNA templates, and also has a higher productivity.

Area of applications Synthesis of cDNA on RNA template
RT-PCR
One step RT-PCR
Preparation of cDNA libraries
Concentration 5 units/μl
Storage conditions from -24°С to -16°С – 2 years
from + 2°С to + 8°С – 3 months
Transportation conditions up to 14 days at a temperature <25°С
Storage and dilution buffer 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA,
100 mМ KCl, 50 % glycerol, 0.5 % Tween-20

E-RTH-50

650 EUR

Size :
50 000 U


Delivery takes up to
5-12 business days

E-RTH-500

5 200 EUR

Size :
500 000 U


Delivery takes up to
5-12 business days