Москва, ул.Годовикова 
Diamant TaqAD DNA Polymerase en
Diamant TaqAD DNA Polymerase
Hot start Taq DNA polymerase with activation temperature above 60°C
Cat. No.
Size, U
Size, ml
Price
To order
* supplied with 10x PCR buffer and 50 mM MgCl2
Benefits:
- Amplification of long PCR fragments up to 5000 bp
- Suitable for one-step RT-PCR
- Suitable for test systems with preamplification
- Reversible hot start9
Area of applications:
- Multiplex PCR
- Routine PCR
- Low copy PCR
- PCR with dual labeled probes
- PCR with intercalating dyes
Storage and dilution buffer: 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50 % glycerol, 0.5 % Tween-20
Concentration:
10 units/μl
Storage and transportation conditions:
from -24°С to -16°С – 2 years
up to 7 days at a temperature <25°С
Diamant TaqAD DNA polymerase is a high-quality hot-start polymerase with superior sensitivity and specificity compared to native Taq polymerase. The reagent is composed of a high-purified recombinant Taq DNA-polymerase and specific low-molecular weight aptamer and antibodies which inhibit enzymatic activity of polymerase at room temperature. According to our tests, polymerase keeps inactive during the incubation with the substrate for about 1 hour at 60°C. Activation of the enzyme starts automatically during the first cycle of DNA denaturation.
| Area of applications | Multiplex PCR Routine PCR Low copy PCR PCR with dual labeled probes PCR with intercalating dyes |
| Concentration | 5 units/μl |
| Storage conditions | from -24°С to -16°С – 2 years from + 2°С to + 8°С – 6 months |
| Transportation conditions | up to 14 days at a temperature <25°С |
| Storage and dilution buffer | 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50 % glycerol, 0.5 % Tween-20 |