Москва, ул.Годовикова 
Diamant taqa dna polymerase en
Diamant TaqA DNA Polymerase
Hot start Taq DNA polymerase with activation temperature above 37°C
Cat. No.
Size, U
Size, ml
Price
To order
* supplied with 10x PCR buffer and 50 mM MgCl2
Benefits:
- Amplification of long PCR fragments up to 5000 bp
- Stable at 2°С – 8°С up to 6 months
Area of applications:
- Multiplex PCR
- Routine PCR
- Low copy PCR
- PCR with dual labeled probes
- PCR with intercalating dyes
Storage and dilution buffer: 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50 % glycerol, 0.5 % Tween-20
Concentration:
5 units/μl
Storage and transportation conditions:
from -24°С to -16°С – 2 years
from + 2°С to + 8°С – 6 months
up to 14 days at a temperature <25°С
Diamant TaqA DNA polymerase is a high quality hot-start polymerase with superior sensitivity and specificity compared to native Taq polymerase. The reagent is composed of a high-purified recombinant Taq DNA polymerase and specific low-molecular weight aptamer which inhibits enzymatic activity of polymerase at room temperature. According to our tests, polymerase keeps inactive during the incubation with the substrate for about 1 hour at 37°C. Activation of the enzyme starts automatically during the first cycle of DNA denaturation. Due to original storage buffer the reagent can be stored at 2-8°C for 6 months without significant changes in activity and inactivation level.
| Area of applications | Multiplex PCR Routine PCR Low copy PCR PCR with dual labeled probes PCR with intercalating dyes |
| Concentration | 5 units/μl |
| Storage conditions | from -24°С to -16°С – 2 years from + 2°С to + 8°С – 6 months |
| Transportation conditions | up to 14 days at a temperature <25°С |
| Storage and dilution buffer | 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50 % glycerol, 0.5 % Tween-20 |