5X One Step RT-PCR Mastermix


Ready-to-use mix for RT-PCR. The reaction is carried out in one tube. Ideal solution for one-step detection of RNA viruses, including SARS-CoV-2

Cat. No.

Reactions (25 uL)

Pack Size

Price

To order

M5-OS-1

1 000 Reactions

4×1,25 ml

750 EUR

M5-OS-10

10 000 Reactions

50 ml

6 200 EUR

Benefits:

  • High sensitivity (single copies of RNA)
  • Synthesis of cDNA and PCR is carried out in one tube
  • Increased inhibition resistance

Area of applications:

  • Multiplex RT-PCR
  • Routine RT-PCR
  • Low-copy RT-PCR
  • RT-PCR with dual-labeled probes

Storage and transportation conditions:

from -24°С to -16°С – 2 years

up to 7 days at a temperature <25°С

5X One Step RT-PCR mastermix is a ready to use universal reagent for the synthesis of cDNA on RNA matrix with the following quantitative and/or qualitative polymerase chain reaction with real time identification of products (RT-PCR). Synthesis of cDNA and qPCR assay is carried out in one tube. The reagent provides reproducible results with high sensitivity (single copies of RNA) in RT-PCR.
5X One Step RT-PCR mastermix contains all the necessary components for PCR assay, including: «warm start» reverse transcriptase, «hot start» thermostable DNA-polymerase, dNTPs and optimized buffer. Due to some genetic modifications MMLV reverse transcriptase has inhibited RNase H activity, improved thermostability and increased performance with GC-rich RNA matrix, resulting in the higher cDNA yield. «Hot-start» inactivated DNA-polymerase of the Mastermix do not possess any enzymatic activity during PCR-mix preparation and reverse transcription which lead to significant increase of sensitivity and specificity of the assay. Special additives provide increased resistance to inhibitors.

Area of applicationsMultiplex RT-PCR
Routine RT-PCR
Low-copy RT-PCR
RT-PCR with dual-labeled probes
Transportation conditions up to 7 days at a temperature <25°С
Storage conditions from -24°С to -16°С – 2 years

M5-OS-1

750 EUR

Reactions (25 uL):
1 000 Reactions 5 ml


Delivery takes up to
5-12 business days

M5-OS-10

6 200 EUR

Reactions (25 uL):
10 000 Reactions 50 ml


Delivery takes up to
5-12 business days