Москва, ул.Годовикова 
5x fast probe qpcr u mastermix en
5X One Step RT-PCR Mastermix
Ready-to-use mix for RT-PCR. The reaction is carried out in one tube. Ideal solution for one-step detection of RNA viruses, including SARS-CoV-2
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Reactions (25 uL)
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Benefits:
- High sensitivity (single copies of RNA)
- Synthesis of cDNA and PCR is carried out in one tube
- Increased inhibition resistance
Area of applications:
- Multiplex RT-PCR
- Routine RT-PCR
- Low-copy RT-PCR
- RT-PCR with dual-labeled probes
Storage and transportation conditions:
from -24°С to -16°С – 2 years
up to 7 days at a temperature <25°С
5X One Step RT-PCR mastermix is a ready to use universal reagent for the synthesis of cDNA on RNA matrix with the following quantitative and/or qualitative polymerase chain reaction with real time identification of products (RT-PCR). Synthesis of cDNA and qPCR assay is carried out in one tube. The reagent provides reproducible results with high sensitivity (single copies of RNA) in RT-PCR.
5X One Step RT-PCR mastermix contains all the necessary components for PCR assay, including: «warm start» reverse transcriptase, «hot start» thermostable DNA-polymerase, dNTPs and optimized buffer. Due to some genetic modifications MMLV reverse transcriptase has inhibited RNase H activity, improved thermostability and increased performance with GC-rich RNA matrix, resulting in the higher cDNA yield. «Hot-start» inactivated DNA-polymerase of the Mastermix do not possess any enzymatic activity during PCR-mix preparation and reverse transcription which lead to significant increase of sensitivity and specificity of the assay. Special additives provide increased resistance to inhibitors.
| Area of applications | Multiplex RT-PCR Routine RT-PCR Low-copy RT-PCR RT-PCR with dual-labeled probes |
| Transportation conditions | up to 7 days at a temperature <25°С |
| Storage conditions | from -24°С to -16°С – 2 years |