5X Fast probe qPCR U Mastermix


Ready-to-use mastermix for qPCR with UDG to prevent false positives results

Cat. No.

Reactions (25 uL)

Pack Size

Price

To order

M5-FPU-10

10 000 Reactions

50 ml

2 540 EUR

M5-FPU-50

50 000 Reactions

250 ml

10 180 EUR

Benefits:

  • High sensitivity (single copies of DNA)
  • Increased inhibition resistance
  • Prevention of false-positive results
  • Stable at temperatures of + 2 °C – + 8 °C

Area of applications:

  • Multiplex PCR
  • Routine PCR
  • Low-copy PCR
  • PCR with dual-labeled probes

Storage and transportation conditions:

from -24°С to -16°С – 2 years
from + 2°С to + 8°С – 14 months

up to 7 days at a temperature <25°С

5X Fast Probe qPCR U Mastermix is a ready-to-use reagent for real time polymerase chain reaction (qPCR). The reagent contains the following components: thermostable hot start DNA polymerase, dNTPs (including dUTP), uracil-DNA glycosylase (UDG), PCR optimized buffer. The use of inactivated DNA polymerase, which does not possess enzymatic activity under the conditions of preparation of the PCR mixture, leads to an increase in the specificity and sensitivity of the reaction. Enzyme activation occurs automatically during the first cycle of DNA denaturation. The presence of dUTP in the reaction mixture leads to the fact that all amplification products will contain uracil. In this case, UDG effectively hydrolyzes uracil from single- and double-stranded DNA molecules during the preparation of the reaction, thus preventing the amplification of the products of previous PCR (amplicons), which help to avoids false-positive results. UDG is completely inactivated during PCR and does not interfere with the amplification of the products of the current reaction. Special additives provide increased resistance to inhibitors. Mastermix can also contain ROX or dyes and other reagents for loading resulting PCR mixture on gel.

Area of applicationsMultiplex PCR
Routine PCR
Low-copy PCR
PCR with dual-labeled probes
Transportation conditions up to 7 days at a temperature <25°С
Storage conditions from -24°С to -16°С – 2 years
from + 2°С to + 8°С – 14 months

M5-FPU-10

2 540 EUR

Reactions (25 uL):
10 000 Reactions 50 ml


Delivery takes up to
5-12 business days

M5-FPU-50

10 180 EUR

Reactions (25 uL):
50 000 Reactions 250 ml


Delivery takes up to
5-12 business days