5X Fast probe qPCR U Mastermix EN

5X Fast Probe qPCR U Mastermix is a ready-to-use reagent for real time polymerase chain reaction (qPCR). The reagent contains the following components: thermostable hot start DNA polymerase, dNTPs (including dUTP), uracil-DNA glycosylase (UDG), PCR optimized buffer. The use of inactivated DNA polymerase, which does not possess enzymatic activity under the conditions of preparation of the PCR mixture, leads to an increase in the specificity and sensitivity of the reaction. Enzyme activation occurs automatically during the first cycle of DNA denaturation. The presence of dUTP in the reaction mixture leads to the fact that all amplification products will contain uracil. In this case, UDG effectively hydrolyzes uracil from single- and double-stranded DNA molecules during the preparation of the reaction, thus preventing the amplification of the products of previous PCR (amplicons), which help to avoids false-positive results. UDG is completely inactivated during PCR and does not interfere with the amplification of the products of the current reaction. Special additives provide increased resistance to inhibitors. Mastermix can also contain ROX or dyes and other reagents for loading resulting PCR mixture on gel.