5x fast probe qpcr mastermix en
5X Fast Probe qPCR Mastermix

Ready-to-use mastermix for qPCR
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Reactions (25 uL)
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Benefits:
- High sensitivity (single copies of DNA)
- Increased inhibition resistance
- Stable at temperatures of + 2 °C – + 8 °C
Area of applications:
- Multiplex PCR
- Routine PCR
- Low-copy PCR
- PCR with dual-labeled probes
Storage and transportation conditions:
from -24°С to -16°С – 2 years
from + 2°С to + 8°С – 14 months
up to 7 days at a temperature <25°С
5X Fast Probe qPCR Mastermix is versatile, ready-to-use reagent for real time polymerase chain reaction (qPCR). The use of the reagent provides reproducible results with high sensitivity (single copies of DNA) when determining the amount of genomic, plasmid, viral and cDNA by qPCR. Mastermix contains all the necessary components for PCR, including: thermostable hot start DNA-polymerase, dNTP and optimized buffer. The mastermix contains inactivated DNA polymerase, which does not exhibit enzymatic activity under the conditions of preparation of the PCR mixture, which leads to an increase in the specificity and sensitivity of the reaction as compared to conventional Taq polymerase. Special additives provide increased resistance to inhibitors. Mastermix can also contain ROX or dyes and other reagents for loading resulting PCR mixture on gel.
Area of applications | Multiplex PCR Routine PCR Low-copy PCR PCR with dual-labeled probes |
Transportation conditions | up to 7 days at a temperature <25°С |
Storage conditions | from -24°С to -16°С – 2 years from + 2°С to + 8°С – 14 months |